Production, characterization, and optimization of rhamnolipids produced by Pseudomonas aeruginosa by solid-state fermentation

Abstract

Background: Pseudomonas aeruginosa that produces biosurfactant (rhamnolipids) is used for emulsifying the oil-infested areas. The objective of the study was to isolate and identify a strain of environmentally important that can act on oil-contaminated soil. Materials and Methods: Isolation and identification of P. aeruginosa from oil‑spilled soil isolated from Chennai. Rhamnolipid production was enhanced by fish oil. pH, temperature and incubation time were optimize using RSM, solid-state fermentation (sugarcane bagasse) was adopted for production of rhamnolipid. Results: The isolated strain of P. aeruginosa was subjected to optimization of cultural condition such as temperature, pH, and incubation time representing 35°C, 6, and 5 days, respectively. The solid-state fermentation was done to find the enzyme production using molasses and fish oil. The surfactant that was extracted was analyzed for surface tension ability to confirm the used as biodetergents. Discussion and Conclusion: From the above analysis, P. aeruginosa could be ideal candidate for the production of biosurfactant that can be used in the environmental oil slippage.