Hypoglycemic effect of ethanolic extract of bark of Terminalia arjuna Linn. in normal and alloxan‑induced noninsulin-dependent diabetes mellitus albino rats
DOI:
https://doi.org/10.22377/ijgp.v6i4.274Abstract
Introduction: The Bark of Terminalia arjuna Linn. (family‑Combretaceae) is used in Indian Ayurvedic medicine for the treatment of diabetes mellitus. Aim: Therefore, the effect of ethanolic extract of bark of Terminalia arjuna on blood glucoselevel of normal and diabetic rats was evaluated in this study. Materials and Methods: Healthy Wistar albino rats (100-150
gm) were divided into four groups of six animals each. Normal control group received normal saline (10 ml/kg/day/p.o.);
diabetic treated group received ethanolic extract of bark of Terminalia arjuna (500 mg/kg/p.o.); diabetic standard group
received Glibenclamide (0.5 mg/kg/day/p.o.) given for 2 weeks. To induce diabetes, alloxan 150 mg/kg, i.p. single dose
was administered to diabetic control, diabetic treated and diabetic standard groups. Blood glucose and body weight was
estimated weekly for two weeks. For mechanism of action glycogen estimation on liver, cardiac and skeletal muscle; effect
on adrenaline induced hyperglycemia and intestinal glucose absorption was done. For hypoglycemic action on normal rats,
blood glucose was estimated at ‘0’ min and ‘120’ min. Results: The test drug showed significant decrease (P<0.01) in blood
glucose level. The test drug showed a significant (P<0.01) increase in glycogen content in liver, cardiac and skeletal muscle,
significantly (P<0.01) reduced adrenaline induced hyperglycemia and intestinal glucose absorption. Blood glucose in normal
rats was significantly (P<0.01) decreased in drug treated groups. Conclusion: Terminalia arjuna bark possesses significant
hypoglycaemic and anti‑diabetic activity.
Key words: Antioxidant, blood glucose, flavonoids, glycogen
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References
Jung HW, Jung JK, Ramalingam M, Yoon C, Bae HS, Park Y.
Anti‑diabetic effect of Wen‑pi‑tang‑Hab‑Wu‑ling‑san extract
in streptozotocin‑induced diabetic rats. Indian J Pharmacol
;44:97‑102.
Powers AC. Diabetes Mellitus. In: Longo DL, Kasper DL,
Jameson JL, Fauci AS, Hauser SL, Loscalzo J, editors. Harrison’s
principles of Internal Medicine. 18th ed. New Delhi: McGraw‑Hill
Medical publishing division; 2012. p. 2968‑3002.
Davis SN. Insulin, oral hypoglycemic agents, and the pharmacology
of the endocrine pancreas. In: Brunton LL, Lazo JS, Parker KL,
editors. Goodman and Gilman’s the pharmacological basis of
therapeutics. 11th ed. New York: McGraw–Hill, Medical Publishing
Division; 2006. p. 1613‑45.
Umashanker M, Shruti S. Traditional Indian herbal medicine used
as antipyretic, antiulcer, anti‑diabetic and anticancer: A review. Int
J Res in Pharm and Chem 2011;1:1152‑9.
Kumar DS, Prabhakar YS. On the ethnomedical significance
of the arjun tree, Terminalia arjuna (Roxb.) Wight and Arnot.
J Ethnopharmacol 1987;20:173‑90.
Terminalia arjuna Extract. Mother herbs and agro product. Available
from: http://www.motherherbs.com/terminalia‑arjuna‑extract.
html. [Last accessed on 2012 Aug 29]
Nairn JG. Solutions, emulsions, suspensions and extracts.
In: Gennaro A, Marderosian AD, Hanson GR, Medwick T,
Popovich NG, Schnaare RL, Schwartz JB, White HS,
editors. Remington: The science and practice of pharmacy. 20th ed.
Philadelphia: Lippincott Williams and Wilkins; 2000. p. 721‑52.
Organization for Economic Cooperation and Development (OECD).
OECD Guidelines for Testing of Chemicals [Internet]. France:
OECD Publishing; 2006 July 11. Section 4, Health Effects: Test
No. 425: Acute Oral Toxicity: Up‑and‑Down Procedure. Available
from: http://www.oecdbookshop.org/oecd/index.asp/langen. [Last
accessed on 2009 Mar 22].
Ghosh R, Sharatchandra K, Rita S, Thokchom IS. Hypoglycemic
activity of Ficus hispida (bark) in normal and diabetic albino rats.
Indian J Pharmacol 2004;36:222‑5.
Ghosh MN. Toxicity Studies. Fundamentals of Experimental
Pharmacology. 4th ed. Kolkata: Hilton and Company; 2005. p. 176‑89.
Sood Ramnik. Clinical chemistry, blood sugar estimation. Medical
laboratory technology methods of interpretations. New Delhi:
Japyee Brothers Medical Publishers (P) Ltd; 5th ed. 1999. p. 433‑6.
Akhtar MA, Rashid M, Wahed MI, Islam MR, Shaheen SM,
Islam MA, et al. Comparison of long–term antihyperglycemic and
hypolipidemic effects between Coccinia cordifolia (Linn.) and
Catharanthus roseus (Linn.) in alloxan–induced diabetic rats. Res
J Med and Med Sci 2007;2:29‑34.
Kulkarni SK. Commonly used drugs, their doses and nature
of action in laboratory animals. Handbook of Experimental
Pharmacology. 3rd ed. Delhi: Vallabh Prakashan; 2005. p. 190‑5.
Caroll NV, Longley RW, Roe JH. The determination of glycogen
in liver and muscle by use of anthrone reagent. J Biol Chem
;200:583‑93.
Anturlikar SD, Gopumadhavan S, Chauhan BL, Mitra SK.
Effect of D–400, a herbal formulation on blood sugar of normal
and alloxan induced diabetic rats. Indian J Physiol Pharmacol
;39:95‑100.
Das S, Jadav RK, Nagchoudhuri J. Effect of fasting on the intestinal
absorption of D‑glucose and D‑xylose in rats in vivo. Indian J
Physiol Pharmacol 2001;45:451‑6.
Kaneto H, Kajimoto Y, Miyagawa J, Matsuoka T, Fujitani Y,
Umayahara Y, et al. Beneficial effects of antioxidants in diabetes:
Possible protection of pancreatic b–cells against glucose toxicity.
Diabetes 1999;48:2398‑406.
Szkudelski T. The mechanism of alloxan and streptozotocin action
in B cells of the rat pancreas. Physiol Res 2001;50:536‑46.
El–Soud NA, Khalil MY, Hussein JS, Oraby FH, Farrag AH.
Antidiabetic effects of Fenugreek alkaloid extract in streptozotocin
induced hyperglycemic rats. J Appl Sci Res 2007;3:1073‑83.
Hakkim FL, Girija S, Kumar RS, Jalaluddeen MD. Effect of aqueous
and ethanol extracts of Cassia auriculata L. flowers on diabetes
using alloxan induced diabetic rats. Int J Diabetes and Metabolism
;15:100‑6.
Tiwari AK, Rao JM. Diabetes mellitus and multiple therapeutic
approaches of phytochemicals: Present status and future prospects.
Curr Sci 2002;83:30‑8.
Chander R, Singh K, Khanna AK, Kaul SM, Puri A, Saxena R,
et al. Antidyslipidemic and antioxidant activities of different
fractions of Terminalia arjuna stem bark. Indian J Clin Biochem 2004;
:141‑8.
Anjaneyulu AS, Prasad AVs. Chemical examination of roots of
Terminalia arjuna (Roxb.) Wight and Arnot: Part 1‑characterisation
of two new triterpinoid glycosides. Indian J Chem 1982;
B: 530‑3.
DeFronzo RA. Pathogenesis of type 2 diabetes mellitus. Med Clin
North Am 2004;88:787‑835.
Kumar GP, Arulselvan P, Kumar DS. Antidiabetic activity of fruits
of Terminalia chebula on streptozotocin induced diabetic rats.
J Health Sci 2006;52:283‑91.
Tripathi KD. Adrenergic Systems and Drugs: Essentials of Medical
Pharmacology. 5th ed. Jaypee Brothers Medical Publisher Ltd; 2004.
p. 103‑19.
