Effects of ethanol extract of Pisonia aculeata Linn. on ehrlich ascites carcinoma tumor bearing mice
DOI:
https://doi.org/10.22377/ijgp.v2i1.397Abstract
In order to scientifi cally appraise some of the anecdotal, folkloric, ethno medical uses of Pisonia aculeata Linn. (Nyctaginaceae), the present study was undertaken to examine the antitumor activity of Pisonia aculeata leaves extract on Ehrlich Ascites Carcinoma (EAC) in mice. Tumor was induced in mice by intraperitoneal injection of Ehrlich Ascites Carcinoma cells (1X106 cells/mouse). Ethanol extract of Pisonia aculeata (EEPA) was administered to the experimental animals at the doses of 250 & 500 mg/kg/day, p.o. Th e antitumor effect of the extract was evaluated by using survival time, hematological parameters, increase in body weight, solid tumour volume and peritoneal cell count. Oral administration of EEPA increased the survival time and inhibits the weight gain of the tumor bearing mice. After 14 days of inoculation, the extract also reduces the solid tumor volume developed by the EAC cells. Th e fi ndings of thisstudy indicate that the EEPA possesses signifi cant antitumor activity on dose dependent manner..
Key words: Pisonia aculeata, ehrlich ascites carcinoma, hematological parameters, survival time, peritoneal cell count and solid tumor
Downloads
References
Anonymous., The wealth of India – Raw Materials, Vol. VIII,
Council for ScientiÞ c and Industrial Research, New Delhi, 2003,
Brown J.P., A review of the genetic eff ect of naturally occurring
ß avonoids, anthraquinones and related compounds, Mutat Res,
, 1980, 243-247.
Clarkson B.D., Burchenal J.H., Preliminary screening of
Table 4. Effect of EEPA on solid tumor volume
Design of Solid tumor volume (ml)
treatment 15th day 20th day 25th day 30th day
Tumor control 6.7 ± 0.51 8.28 ± 0.3 10.88 ± 0.3 12.12 ± 0.4
EEPA (250 mg 4.33 ± 0.33b 5.56 ± 0.3b 7.12 ± 0.1a 7.34 ± 0.33b
/kg)
EEPA (500 mg 5.63 ± 0.21a 5.88 ± 0.16a 5.88 ± 0.21a 6.55 ± 0.1a
/kg)
N = 6 animals in each group, aP <0.001; bP <0.01 when compared with control, Values
are expressed as mean ± SEM.
antineoplastic drugs, Prog Clin Cancer, 1, 1965, 625-629.
DÂ’Amour F.F., Blood F.R., Belden D.A., The Manual for Laboratory
Work in Mammalian Physiology, The University of Chicago Press,
Chicago, 1965, 148-150.
Docie J.V., Practical Haemotology, Edn. 2, J&A Churchill Ltd,
London, 1958, 38-42.
Fotsis T., Pepper M.S., Aktas E., Breit S., Rasku S., Adlercreutz H.,
Flavonoids, dietery-derived inhibitors of cell proliferation and in
vitro angiogenesis, Cancer Res, 57, 1997, 2916-2921.
Gothoskar S.V., Ranadive K.J., Anticancer screening of SAN-AB:
An extract of marking nut Semicarpus anacardium, Indian J Exp
Biol, 9, 1971, 372-375.
Hirano T., Oka K., Akiba M., Antiproliferative eff ect of synthetic
and naturally occurring ß avonoids on tumor cells of human
breast carcinoma cell lines, ZR-75-1, Res Commun Chem Pathol
Pharmacol, 64, 1989, 69-78.
Jin G., You Y.,Ahn B., Esters of 2-(1-hydroxyalkyl)-1,4-dihydroxy-
,10-anthraquinones with melphalan as multifunctional anticancer
agents, Bioorg Med Chem Lett , 11, 2001, 1473-1476.
Kutt an G., Vasudevan D.M., Kutt an R., Eff ect of a preparation
from Viscum album in tumour development in vitro and in mice,
J Ethnopharmacol, 29, 1990, 35-41.
Lowry O.H., Rosenbrough N.T., Farr A.L., Protein measurement
with Folin – Phenol reagent, J Biol Chem, 173, 1951, 265-275.
Nadkarni A.K., Indian Materia Medica, Vol. 1, Popular Prakashan,
Bombay, 2005, 972-973.
Oberling C., Guerin M., The role of viruses in the production of
cancer, Advances in Cancer Research II, Academic Press, New
York, 1954, 406-410.
Ramnath V., Kutt an G., Kutt an R., Antitumour eff ect of abrin on
transplanted tumours in mice. Indian J Physiol Pharmacol, 46,
, 69-77.
Suff ness M., Douros J., In: Devita V.T., (edr.) Methods in cancer
research, Academic Press, New York, 1978, 73-75.
Sur P., Ganguly D.K., Tea plant root extract (TRE) as an
antineoplastic agent, Planta Med, 60, 1994, 106-109.
Tomlinson S.K., Melin S.A., Higgs V., White D.R., Savage P., Case
D., Blockstock A.W., Schedule selective biochemical modulation
of 5-ß uorouracil in advanced colorectal cancer – a phase II study
, BMC Cancer , 2, 1990, 9.
Wagner H., Bladt S., Zgainski E.M., Plant drug analysis, Berlin,
Heidelberg, New York, Tokyo: Springer-Verlag, 1984, 298-34.
Weber G., Shen F., Prajda N., Yeh Y.A., Yang H., Herenyiova,
Increased signal transduction activity and down regulation in
human cancer cells, Anticancer Res, 16, 1996, 3271-3282.
